Michael Hooker Microscopy Facility

The Hooker Microscopy Facility is located in the UNC School of Medicine

Booking Calendars - see cal.confocal.org:88

At the University of North Carolina

Notices

Notices:

  • The Olympus VS200 Slide Scanner is unavailable due to a controller malfunction
  • May 2022 - NAC is no longer required and may be uninstalled
    • Hygiene Quick Guide:
      • UNC Employee daily Wellness Check
      • Wash hands with soap and water before and after using a system.
      • Fresh disposable gloves are OK. Please bring your own. Dispose of gloves when leaving area.
      • 70% alcohol is for gently wiping down with paper towel (not spraying) the keyboard, stage, mouse, joy stick and counter top. Never spray near or on the microscope!
      • Microscope rubber parts can be cleaned with 70% isopropyl alcohol.
      • On scopes with eye pieces remove rubber cups and use spectacles or light weight safety goggles.
      • Dispose of wipes. DO NOT leave them on the bench.
      • Take slides, boxes, etc. with you when done so that bench area can be easily wiped down.
    Michael Hooker Microscopy Facility web site - historical and informational  

     

  • Notices:

  • May 2022 - NAC is no longer required and may be uninstalled
  •  
     
     

    Location

    • Thurston Bowles building room 6024
    • Marsico Hall 7th floor

    Contacting MHMF

    • Michael 919-912-9380  voice/text

    Booking Time on a Resource

     
    The Olympus VS200 slide scanner is unavailable due to a controller error. System is being assessed Aug 1

    Publications 2002 - 2014



    Confocal First light - Mixed pollen grains 
     

    5 Channels - CY2  CY3  CY3.5  CY5  CY7

    Excitation - White Light Laser 20x 0.75NA


    Magenta Darkfield
    Yellow Confocal reflection
    Blue DAPI
    Green Fluorescence
    40x 1.25 NA adjusted to ~0.7

    Spheroid
    Confocal scan 40x NA 0.6
    Spheroid
    DIC

     
     

    Copyright 2001-2022  M. Chua  -  School of Medicine, University of North Carolina, Chapel Hill, NC 27599

    Last updated  08/01/22